ACUTE TOXICITY AND ANTINOCICEPTIVE ACTIVITY OF SAPONINS RICH FRACTION OF DIOSCOREA DELTOIDEA (WALL)

Main Article Content

Niaz Ali
Nur- Ul- Ain
Muhammad Nabi
Zakia Subhan
Shakir Ullah
Ulfat Sultana
Bilal Shams

Abstract

OBJECTIVE: To evaluate the saponins rich fraction of Dioscorea deltoidea (D. deltoidea) for possible antinociceptive activity.
METHODS: Saponins were extracted from the crude methanolic extract of D. deltoidea (Wall). Presence of saponins was confirmed through phytochemical screenings. Acute toxicity test was performed to determine safe dose range of the saponins rich fraction (n-butanol fraction) using balb C Mice. The saponins rich fraction was assessed for possible antinociceptive activity using acetic acid induced writhing method and hot plate method. Data was analyzed using Graph Pad Prism 6. ANOVA was used to determine the significance of test samples versus positive control at 95% CI with p<0.05.
RESULTS: The results showed that crude saponin rich fraction was safe up to test dose of 1000 mg/kg administered orally. In acetic acid induced writhing method, its test samples in doses of 10 and 50 mg/kg showed respectively 75.51% and 85.71% inhibition of writhing, while diclofenac sodium showed 74.4% inhibition of writhing. Percent inhibition of latency time of test samples increased from 87.87% to 133.6% in test doses of 10 mg/kg to 100 mg/kg dose, respectively, while Tramadol showed latency time of 85.12% within 30 minutes of its administration.
CONCLUSION: Saponins rich n-butanol fraction of Dioscorea deltoidea (D.deltoidea) showed significant antinociceptive activity.

Article Details

How to Cite
Ali, Niaz, et al. “ACUTE TOXICITY AND ANTINOCICEPTIVE ACTIVITY OF SAPONINS RICH FRACTION OF DIOSCOREA DELTOIDEA (WALL)”. KHYBER MEDICAL UNIVERSITY JOURNAL, vol. 12, no. 2, June 2020, pp. 107-12, doi:10.35845/kmuj.2020.19787.
Section
Original Articles

References

Serpell M. Anatomy, physiology and pharmacology of pain. Surgery 2006;24(10):350-3. DOI: 10.1053/j.mpsur.2006.08.002.

Shoaib M, Shah SWA, Ali N, Shah I, Ullah S, Ghias M, et al. Scientific investigation of crude alkaloids from medicinal plants for the management of pain. BMC Complement Altern Med 2016;16(1):178. DOI: 10.1186/s12906-016-1157-2

Malairajan P, Gopalakrishnan G, Narasimhan S, Veni KJK. Analgesic activity of some Indian medicinal plants. J Ethnopharmacol 2006;106(3):425-8. DOI: 10.1016/j.jep.2006.03.015

Khan MA, Rahman AA, Nahar L, Islam MB, Alam AK. In vivo analgesic and CNS depressant activities of antioxidative stem bark fraction of Morus alba L. J Pharm Sci 2014;13(2):225-7. DOI: 10.3329/dujps.v13i2.21905.

Dutta B. Food and medicinal values of certain species of Dioscorea with special reference to Assam. J Pharmacogn Phytochem 2015;3(4):15-8

Dangwal L, Chauhan AS. Dioscorea deltoidea wall. Ex Griseb. A Highly Threatened Himalayan Medicinal Plant: An Overview. Int J Pharm Bio Sci 2015;6(1): 452-460.

Tahir N, Bibi Y, Iqbal M, Hussain M, Laraib S, Safdar I, et al. Overview of Dioscorea deltoidea Wall. Ex Griseb: An Endangered Medicinal Plant from Himalaya Region. J Biodivers Environ Sci 2016;9(6):13-24.

Chandra S, Saklani S, Mishra AP. In vitro Antimicrobial Activity of Garhwal Himalaya Medicinal Plant Dioscorea deltoidea Tuber. Int J Herb Med 2013;1(4):67-70.

Hamayun M. Traditional uses of some medicinal plants of Swat Valley, Pakistan. 2007. Indian J Traditional Knowledge 2007;6:636-41.

Muslim M. Sikander S. Ethnobotany of medicinal plants of Leepa Valley (Azad Kashmir). Pak J Forestry 2010;60(2):21-30.

Mustafa A, Ahmad A, Tantray AH, Parry PA. Ethnopharmacological Potential and Medicinal Uses of Miracle Herb Dioscorea spp. J Ayu Her Med 2018;4(2):79-85.

Priti K, Joshi GC, Tewari LM. Indigenous uses of threatened ethno-medicinal plants used to cure different diseases by ethnic people of Almora district of western Himalaya. Int J Ayurvedic Herb Med 2012;2(04):661-78.

Razaq A, Rashid A, Ali H, Ahmad H, Islam M. Ethnomedicinal potential of plants of Changa Valley District, Shangla, Pakistan. Pakistan J Bot 2010;42(5):3463-75.

Asha K, Nair GM. Screening of Dioscorea species for diosgenin from southern Western Ghats of India. Indian J Plant Genet Resour 2005;18(2):227-30.

Sharma V, Ramawat KG. Tuberous Medicinal Plants of India. In: Ramawat KG, Merillon J Bulbous Plants. 1st ed. India: CRC Press; 2013.p 311-37.

Güçlü-Üstündağ Ö, Mazza G. Saponins: properties, applications and processing. Crit Rev Food Sci Nutr 2007;47(3):231-58. DOI: 10.1080/10408390600698197

Chindo B, Anuka J, Isaac E, Ahmadu A, Tarfa F, Gamaniel K. Saponins are involved in the analgesic and anti-inflammatory properties of Ficus platyphylla stem bark. Int J Biol Chem Sci 2010;4(2):415-23.

Borgi W, Recio M-C, Ríos J, Chouchane N. Anti-inflammatory and analgesic activities of flavonoid and saponin fractions from Zizyphus lotus (L.) Lam. S Afr J Bot 2008;74(2):320-4. DOI: 10.1016/j.sajb.2008.01.009.

Gyawali R. Antiinflammatory and Antimicrobial Property of Dioscorea deltoidea L from Nepal. Int J Pharm Biol Arch 2015;5(4).

Sharp P, Villano JS. The laboratory rat. 2nd ed: CRC press;2012.

Ali N, Alam H, Khan A, Ahmed G, Shah WA, Nabi M, et al. Antispasmodic and antidiarrhoeal activity of the fruit of Rosa moschata (J). BMC Complement Altern Med 2014;14(1):485. DOI: 10.1186/1472-6882-14-485

Mir MA, Parihar K, Tabasum U, Kumari E. Estimation of alkaloid, saponin and flavonoid, content in various extracts of Crocus sativa. J Med Plants Stud 2016;4(5):171-4.

Ali N, Shah I, Shah SWA, Ahmed G, Shoaib M, Junaid M, et al. Antioxidant and relaxant activity of fractions of crude methanol extract and essential oil of Artemisia macrocephala jacquem. BMC Complement Altern Med 2013;13(1):96. DOI: 10.1186/1472-6882-13-96.

Ali N, Ahmed G, Shah SWA, Shah I, Ghias M, Khan I. Acute toxicity, brine shrimp cytotoxicity and relaxant activity of fruits of callistemon citrinus curtis. BMC Complement Altern Med 2011;11(1):99. DOI: 10.1186/1472-6882-11-99.

Ullah HA, Zaman S, Juhara F, Akter L, Tareq SM, Masum EH, et al. Evaluation of antinociceptive, in-vivo & in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC Complement Altern Med 2014;14(1):346. DOI: 10.1186/1472-6882-14-346.

Afsar T, Khan MR, Razak S, Ullah S, Mirza B. Antipyretic, anti-inflammatory and analgesic activity of Acacia hydaspica R. Parker and its phytochemical analysis. BMC Complement Altern Med 2015;15(1):136. DOI: 10.1186/s12906-015-0658-8.

Muhammad N, Saeed M, Khan H. Antipyretic, analgesic and anti-inflammatory activity of Viola betonicifolia whole plant. BMC Complement Altern Med 2012;12(1):59. DOI: 10.1186/1472-6882-12-59.

Shoaib M, Shah I, Ali N, Shah WA. A mechanistic approach to anti-nociceptive potential of Artemisia macrocephala J acquem. BMC Complement Altern Med 2016;16(1):141. DOI: 10.1186/s12906-016-1114-0.

Ali N, Sultana U, Shah SWA, Nabi M, Shah I, Ahmed G. Acute toxicity and analgesic activity of crude flavonoids of achillea wilhelmsii and teucrium stocksianum. Khyber Med Univ J 2016;8(1):7-11. DOI: 10.35845/kmuj.2016.15844.

Zulfiker A, Rahman MM, Hossain MK, Hamid K, Mazumder M, Rana MS. In vivo analgesic activity of ethanolic extracts of two medicinal plants-Scoparia dulcis L. and Ficus racemosa Linn. Biol Med 2010;2(2):42-8.

Gawade S. Acetic acid induced painful endogenous infliction in writhing test on mice. J Pharmacol Pharmacother 2012;3(4):348. DOI: 10.4103/0976-500X.103699.

Islam T, Begum A, Shahed-Al-Mahmud. Antinociceptive Activity of Methanol Extract of Dioscorea Pentaphylla Linn. Leaves in Mice. Adv in Pharmacol Pharm 2018;6(3):65-71. DOI: 10.13189/app.2018.060301

Lima CM, Lima AK, Melo MGD, Serafini MR, Oliveira DL, de Almeida EB, et al. Bioassay-guided evaluation of Dioscorea villosa–an acute and subchronic toxicity, antinociceptive and anti-inflammatory approach. BMC Complement Altern Med 2013;13(1):195. DOI: 10.1186/1472-6882-13-195.

Nguelefack TB, Dutra RC, Paszcuk AF, Andrade EL, Tapondjou LA, Calixto JB. Antinociceptive activities of the methanol extract of the bulbs of Dioscorea bulbifera L. Var sativa in mice is dependent of NO–cgmp–ATP-sensitive-K+ channel activation. J. Ethnopharmacol 2010;128(3):567-74. DOI: 10.1016/j.jep.2010.01.061.

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