HISTOLOGIC EFFECTS OF ISONIAZID ON THE LIVER OF ALBINO MICE
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Abstract
ABSTRACT
OBJECTIVE: to investigate the effects of thirty days of isoniazid administration on histomorphology of adult male albino mice liver.
METHODS: Forty healthy adult male mice were obtained from the Veterinary Research Institute Lahore and were kept in the animal house facility of University of Health Sciences Lahore. Animals were fed on normal mouse diet and water ad libitum. Mice were divided into two groups, i.e. group A & B. Group A served as a control and received 10ml/kg of distilled water while group B served as experimental and treated with isoniazid 100mg/kg body weight for 30 days. At the end of the experiment liver was dissected and processed for light microscopy. Serial sections of liver 4µm thick were stained with Harris Hematoxylin & Eosin and Periodic Acid Schiff (PAS) for microscopic examination.
RESULTS: No obvious gross abnormalities were present in either control or experimental group. Group A showed the normal liver histology. Group B showed disturbed liver architecture with loss of radial arrangement of hepatic cords and sinusoids. Hepatocyte boundaries were ill-defined. Darkly stained nuclei indicating pyknotic changes. Necrosis evident by pyknosis, karyolysis and karyorrhexis was also observed. Apoptotic bodies were clearly seen and fatty change was evident by signet ring formation. Larger, swollen and empty looking hepatocytes, with the loss of cytoplasmic contents appearing as micro and macro vacuoles were noticed. Vessels were dilated and congested. Periportal and focal areas of inflammation were present.
CONCLUSION: Isoniazid treatment has potential hepatotoxic effects on male albino mice causing disturbed liver architecture.
Key words: Isoniazid (MeSH), Necrosis (MeSH), Apoptosis (MeSH), hepatotoxic (Non-MeSH), Antitubercular Agents (MeSH).
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