ACUTE TOXICITY AND ANALGESIC ACTIVITY OF CRUDE FLAVONOIDS OF ACHILLEA WILHELMSII AND TEUCRIUM STOCKSIANUM
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Abstract
OBJECTIVES: To isolate total flavonoids from Achillea Wilhelmsii and
Teucrium Stocksianum and screen them for possible analgesic activity
and to determine the safe dose range of total flavonoids for preclinical
studies in mice.
METHODS: Acute toxicity studies were performed in mice to determine
its safe dose range for total flavonoids of Achillea wilhelmsii and Teucrium
stocksianum. Writhing tests were performed in mice for possible
analgesic activity. Tail immersion method was used in rats for possible
analgesic activity.
RESULTS: Respective LD50 for crude flavonoids of Achillea wilhelmsii
(CFA) and crude flavonoids of Teucrium stocksianum (CFT) are
1312±12.76 mg/kg and 1248 ±29.6 mg/kg. In acetic acid induced
writhing, analgesic effect of CFA and CFT in dose 10 mg/kg is almost
comparable with standard acetylsalicylic acid (p≤0.05). Similarly, in tail
immersion method, reaction time was significantly prolonged in test
doses of 5 and 10 mg/kg (both for CFA and CFT) suggesting supraspinal
analgesic effect.
CONCLUSION: The results confirm that total crude flavonoids of both
the species, which are safe up to dose of 100 mg/kg, have analgesic
activity. It further warrants for isolation and purification of analgesic
flavonoids for new drug development.
KEY WORDS: Achillea (MeSH), Achillea wilhelmsii (Non-MeSH); Teucrium
stocksianum (Non-MeSH); flavonoids (MeSH), crude flavonoids
(Non-MeSH); Analgesics (MeSH).
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