EFFECTS OF WHOLE-GROUND FLAX SEEDS VERSUS FLAX OIL FOR PESTICIDE RESIDUES-INDUCED NEPHROTOXICITY IN MALE WISTAR RATS
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OBJECTIVES: To observe the possible curative effects of whole-ground flax seeds meal versus flax oil for pesticide residue-induced nephrotoxicity.
METHODS: In this experimental study, 24 male Wistar rats were divided into four equal groups: control group (A) and experimental groups (B, C & D), as approved by ethical committee, Postgraduate Medical Institute, Lahore, Pakistan. Subchronic dose of triazophos-8.2 mg/kg body weight (1/10th of LD50) was given to the group B, C & D via oral gavage, as 1:100 dilution solution for 21 consecutive days. In addition, group C was given flax seeds oil (1.86 g/kg/day) while group D was given whole ground flax seeds meal (800 mg/kg/day). Biochemical analysis of serum creatinine on day 1, 7, 14 and 21 was done. Hematoxylin and Eosin and Periodic Acid Schiff staining was used for histological examination. The mean diameter of renal corpuscle (RCD), proximal convoluted tubule (PCT) and distal convoluted tubule (DCT) in all groups were calculated by micrometry.
RESULTS: Micrometric measurement in group A were as follows: RCD-89.43±3.43µm, PCT diameter-38.90±2.72µm, DCT diameter-34.48±2.58µm). Group B, C & D slides showed RCD-70.12±2.76µm, 80.67±3.34µm, 87.49±4.66µm), PCT diameters (50.35±2.61µm, 43.70±2.35µm, 38.45±2.57µm) and DCT diameters (48.03±3.42µm, 38.65±2.55µm, 35.33±3.02µm) respectively. Biochemical analysis showed raised serum creatinine in group B (2.183±0.367mg/dL), near normal levels in group C (0.698±0.013mg/dL) & comparable with control group, in group D (0.718±0.051mg/dL).
CONCLUSION: Renal tissue when exposed to pesticides, showed disturbed biochemical and histological architecture. Whole-ground flax seeds ameliorate the histopathological and biochemical renal damage better than flax seeds oil against pesticides-residues exposure.
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