COMPARISON OF OXIDATIVE STRESS MARKERS AND ANTI-OXIDANT DEFENSE SYSTEM IN ASTHAMATIC AND NON-ASTHAMATIC WOMEN DURING SECOND TRIMESTER OF PREGNANCY
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OBJECTIVE: To compare the levels of stress markers and anti-oxidants during the second trimester of pregnancy in healthy and asthmatic women.
METHODS: This cross-sectional research, conducted at Institute of Molecular Biology and Biotechnology, University of Lahore and Pak Red Crescent Teaching Hospital, Dina Nath, from October 2016 to March 2017. One hundred females (50 healthy and 50 asthmatics) of same age group (20-35 years) and duration of pregnancy (second trimester) were included through purposive sampling technique. Oxidative stress markers [Melanodialdehyde (MDA) and Nitric Oxide (NO)], enzymatic [Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Reductase (GSH) and Glutathione Peroxidase (GPx)] and non-enzymatic (Vitamin E, Vitamin C and Vitamin A) anti-oxidants were recorded.
RESULTS: Mean BMI was 21.55±0.35kg/m2 and 20.32±0.25kg/m2 (p-value=<0.001) in healthy and asthmatics respectively. Mean hemoglobin values were 12.21±0.21g/dl and 9.8±0.21g/dl (p-value=<0.001), in oxidative stress marker means, MDA had 1.52±0.75 and 2.86±1.18 (p-value=<0.001), while NO had 12.59±1.91 and 14.91±1.65 (p-value=<0.001) in healthy & asthmatics respectively. In Enzymatic anti-oxidant means, SOD had 0.50±0.19 and 0.15±0.21 (p-value=<0.001), CAT had 3.86±0.59 and 0.02±0.01 (p-value=<0.001), GSH had 9.36±1.01 and 4.27±1.91 (p-value=<0.001), while GPx had 9.48±1.01 and 0.36±0.13 (p-value=<0.001) in healthy & asthmatics respectively. In non- enzymatic anti-oxidant means, Vitamin C showed 0.58±0.18 and 0.38±0.09 (p-value=<0.001), Vitamin E had 0.71±0.13 and 0.34±0.11 (p-value=<0.001), while vitamin A depicted 0.45 ± 0.06 and 0.35 ± 0.11 (p-value=<0.001) in the healthy & asthmatics respectively.
CONCLUSION: Our study suggest that imbalance between reducing and oxidizing systems favors a more oxidative stress in asthmatic women than healthy controls during pregnancy.
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