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Objective: To determine the sensitivity pattern of bacterial isolates in neonatal sepsis at a tertiary care hospital of Peshawar.

Methodology: This cross sectional study was carried out at the department of paediatrics, Rehman medical institute Peshawar, from 1st January 2006 to 30th June 2012. Out of 4900 neonates admitted to the department of neonatology during the study period, 2256 (46.04%) neonates had clinical signs and symptoms of sepsis. Only 440/2256 (19.5%) neonates with positive blood cultures were selected for the study. All the blood cultures were done following a standard protocol.

Results: Out of 440 cases of neonatal sepsis, 309 (70.2%) were males and 131 (29.8%) were females. Mean age of patients was 8.93 ± 8.70 days. Nineteen different micro-organism were isolated in which Staphylococcus Aureus (n=282; 64.1%), Enterococcus fecalis (n=61; 13.9%), Klebsiella pneumonia (n=30; 6.8%) and Escherichia coli (n=25; 5.7%) were most common organism. Gram positive organisms were mostly sensitive to vancomycin, imepenime, ceoftaxime, amikacin and amoxacillin, while gram negative organisms were mostly sensitive to amikacin and imepenime. Overall penicillin and cephalosporin has shown high resistance to gram negative organism, while ampicillin was found to be resistant to both gram positive and gram negative organism.

Conclusion: Staphylococcus Aureus, Enterococcus Fecalis, Klebsiella pneumonia and Escherichia coli are the commonest pathogens in neonatal sepsis in our set up. In era of highly resistant micro-organism, vancomycin, amikacin, cefotaxime, and imepinime may be considered as drug of choice for empirical treatment of neonatal sepsis on the basis of highest sensitivity amongst tested drugs.




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