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Rizwan Faisal
Meena Gul
Attia Anwar


OBJECTIVE: To compare the severity of arthritis induced by low and
high doses of pristane in female Sprague-Dawley rats.
METHODS: The study was conducted in Postgraduate Medical Institute, Lahore. Twenty-four female Sprague-Dawley rats were randomly
divided into three equal groups each consisting of 8 rats (n=8); group A served as healthy control, group B was given 150μl pristane and group C was given 500μl pristane intra-dermally in order to induce arthritis.
The severity of arthritis was evaluated by total leukocyte count (TLC),
neutrophil count, lymphocyte count, clinical score of arthritis and body
weight. TLC, neutrophil and lymphocyte count was taken at day 0 and
15 while clinical score of arthritis and body weight were taken at day 0
and then on every alternate day till day 30.
RESULTS: Development of arthritis in both groups B and C was accompanied by rise in TLC, neutrophil count and clinical score of inflammation while lymphocyte count and body weight was decreased in both groups as compared to group A (p<0.001). In group A the clinical score of inflammation was zero throughout the study period. The score of group B and C, which was initially zero at day 0, increased to 12±1 and 16±1 respectively at day 15. The difference was found to be significant at day 15 when group B and C were compared with group A (p<0.001).
The difference between group B and C was also significant (p<0.001).
CONCLUSION: Evaluation of results supported severe induction of
arthritis with 500μl pristane than 150μl.
KEY WORDS: Arthritis, Rheumatoid (MeSH); Pristane (MeSH); Leukocyte Count (MeSH); Neutrophil Count (Non-MeSH); Lymphocyte
Count (Non-MeSH); Clinical score of inflammation (Non-MeSH); Body
Weight (MeSH).

Article Details

How to Cite
Faisal, R., Gul, M., & Anwar, A. (2016). LOW DOSES OF PRISTANE IN COMPARISON WITH HIGH DOSES IN INDUCTION OF ARTHRITIS IN FEMALE SPRAGUE DAWLEY RATS. KHYBER MEDICAL UNIVERSITY JOURNAL, 8(4), 185. Retrieved from https://www.kmuj.kmu.edu.pk/article/view/16822
Original Articles


1.Michael, 2011. Pristane [online] Available at: http://ebooklibrary.org/article/WHEBN0004184875/Pristane#cite_note-2 [Accessed 14/06/2015].

2.Avigan J, Blumer M. On the origin of pristane in marine organisms. J.Lipid Res1968;9:350-52.

3.Anonumous, 2012. Pristane. Available at:http://www.sigmaaldrich.com/catalog/product/sigma/p2870?lang=en®ion=pk[Accessed 14/06/2015].

4.Calvani N, Caricchio R, Tucci M, Sobel ES, Silvestris F, Tartaglia P, et al. Induction of apoptosis by the hydrocarbon oil pristane: implications for pristane-induced lupus. J Immunol 2005;1:175(7):4777-82.

5.Mohamed MA, Mahmoud MF, Rezk AM. Effect of pentoxifylline and pioglitazone on rheumatoid arthritis induced experimentally in rats. Menoufia Med J 2014;27:766-74.

6.De Franco M, Peters LC, Correa MA, Galvan A, Canhamero T, et al. Pristane-Induced Arthritis Loci Interact with the Slc11a1 Gene to Determine Susceptibility in Mice Selected for High Inflammation. PLoS ONE 9(2): e88302.

7.Brenner M, Gulko PS.The arthritis severity locus Cia5a regulates the expression of inflammatory mediators including Syk pathway genes and proteases in pristane-induced arthritis. BMC Genomics 2012;13:710-18.

8.Hoffmann MH, Hopf R, Niederreiter B, Redl H, Smolen JS, Steiner G. Gait changes precede overt arthritis and strongly correlate with symptoms and histopathologicalevents in pristane-induced arthritis. Arthritis Res Ther 2010;12:1-10.

9.William, 2011. Sea Buckthorn insider. [online] Available at: http://www.seabuckthorninsider.com/health-benefits/joints-and-arthritis [Accessed 02/07/2012].

10.Griffiths MM, Cannon GW, Corsi T, Reese V, Kunzler K. Collagen-induced arthritis in rats. Methods Mol Med. 2007;136:201-14.

11.Moon SJ, Park JS, Woo YJ, Lim MA, Kim SM, Lee SY, et al. Rebamipide suppresses collagen-induced arthritis through reciprocal regulation of th17/treg cell differentiation and hemeoxygenase 1 induction. Arthritis Rheumatol. 2014;66(4):874-85.

12.Swart JF, Roock S, Hofhuis FM, Rozemuller H, Broek T, Moerer P, et al. Mesenchymal stem cell therapy in proteoglycan induced arthritis. Ann Rheum Dis. 2015;74(4):769-77.

13.Faisal R, Chiragh S, Popalzai AJ, Rehman K. Anti inflammatory effect of thymoquinone in comparison with methotrexate on pristane induced arthritis in rats. J Pak Med Assoc 2015;65:519-25.

14.Hou WK, Meng LS, Zheng F, Wen YR, Zhu WH, Jiang CS, et al. Methotrexate ameliorates pristane-induced arthritis by decreasing IFN-γ and IL-17A expressions. J Zhejiang Univ-Sci B. 2011;12(1):40-46.

15.Faisal R, Imran U. Comparative evaluation of thymoquinone and methotrexate in lung inflammation in murine model of rheumatoid arthritis. J Postgrad Med Inst 2015;29(2):88-92.

16.Laragione T, Brenner M, Li W, Gulko PS. Cia5d regulates a new fibroblast-like synoviocyte invasion-associated gene expression signature. Arthritis ResTher2008;10(4):2-14.

17.Faisal R, Shinwari L, Jehangir T. Comparison of the therapeutic effects of thymoquinone and methotrexate on renal injury in pristane induced arthritis in rats. J CollPhysicians Surg Pak 2015; 25(8):597-601.

18. Olofsson P, Lu S, Holmberg J, Song T, Wernhoff P, Pettersson U, Holmdahl R. A comparative genetic analysis between collagen-induced arthritis and pristane-induced arthritis. Arthritis & Rheum.2003;48(8):2332-42.

19. Earp JC, Dubois DC, Molano DS, Pyszczynski NA, Keller CE, Almon RR, et al. Modeling Corticosteroid Effects in a Rat Model of Rheumatoid Arthritis I: Mechanistic Disease Progression Model for the Time Course of Collagen-Induced Arthritis in Lewis Rats. J Pharmacol 2008;326(2): 532-45.

20.Olofsson P, Holmdahl R. Pristane induced arthritis in the rat. Methods Mol Med.2007;136:255-68

21.Gou JP, Backdahl L, Marta M, Mathsson L, Ronnelid J, Lorentzen JC. Profound and paradoxical impact on arthritis and autoimmunity of the rat antigen presenting lectin like receptor complex. Arthitis Rheum. 2008;58(5): 1343-53.

22.Hou W, Meng L, Tian L, Zhu W, Jiang C, Lu S. A systemic comparison between collagen induced arthritis and pristane induced arthritis in Dark Agouti rats. ClinExpRheumatol. 2010;28(4):532-8.

23.Holmberg J, Tuncel J, Yamada H, Shemin L, Olofsson P, Holmdahl R. Pristane, a non-antigenic adjuvant, induces MHC classII-Restricted, arthritogenic T cells in the Rat. J. Immunol. 2006;176:1172-79.

24.Bravo AR, Perez M, Valera MJ. The enhancement of growth of a syngeneic plasmacytoma in BALB/c mice by pristane priming is not due to immunosuppressive effects on antibody-forming cell or mitogen-responsive splenocytes. Immunol Lett.1995;44:41-4.