EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE

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Saima Ahmed
Sualiha Lutfullah
Muhammad Asadullah
Izhar Ahmed

Abstract

Objective: to evaluate the anxiolytic effect of Methanolic extract of Tribulus terrestris (MeTt) in Abino mice.

Methods: Tribulus terrestris leaves were collected at the Botanical Garden of the Hamdard University and were identified and authenticated from Eastern Medicine Department of Hamdard University Karachi. Abino mice (30-35 g) of either sex were obtained from the Animal House of Dr.HMI Institute of Pharmacology and Herbal Sciences, Hamdard University, Karachi. Anxiolytic activity was determined using light/dark (LD) test which is commonly used in rodent for anti-anxiety-like behavior that is based on an approach/avoidance conflict between the drive to explore novel areas and an aversion to brightly lit, open spaces: Total thirty six (N=36) mice were randomly divided into six groups. For each of the model studied (n=6). The groups include controls (vehicle) and standard drugs (Diazepam, Bupirone, 1mg/kg) and three groups of MeTt (50,100 and 200 mg/kg.).

Results: MeTtat doses of 50-200 mg/kg significantly (p<0.05, 0.01) increased the latency of entry into the dark box with peak effect produced at the dose of 50 mg/kg (178±28.8) seconds) compared to control (132±9.1 seconds). The extract at doses of 50-200 mg/kg significantly (p<0.05, 0.01) increased the time spent in the light box with peak effect at the dose of 50 mg/kg (178±28.8 seconds) compared to control (132±9.1 seconds). The effect at this dose was not significantly different from that of diazepam (144.5±34.9seconds).

Article Details

How to Cite
Ahmed, S., S. Lutfullah, M. Asadullah, and I. Ahmed. “EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE”. KHYBER MEDICAL UNIVERSITY JOURNAL, vol. 6, no. 2, Apr. 2014, pp. 69-72, https://www.kmuj.kmu.edu.pk/article/view/12354.
Section
Original Articles
Author Biographies

Saima Ahmed, Assistant professor Faculty of pharmacy. Hamdard universty

I am  Saima Ahmed Assistant Professor of pharmacology at the Faculty of Pharmacy,Hamdard university.Karachi.

Sualiha Lutfullah, Research Associate, Faculty of Pharmacy, Hamdard University, Karachi, Pakistan

Saima Ahmed Assistant Professor of pharmacology at the Faculty of Pharmacy,Hamdard university.Karachi.

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