@article{Ali_Sultana_Ali Shah_Nabi_Ullah_Shah_Ahmad_2016, title={ACUTE TOXICITY AND ANALGESIC ACTIVITY OF CRUDE FLAVONOIDS OF ACHILLEA WILHELMSII AND TEUCRIUM STOCKSIANUM}, volume={8}, url={https://www.kmuj.kmu.edu.pk/article/view/15844}, abstractNote={<p><strong>OBJECTIVES</strong>: To isolate total flavonoids from Achillea Wilhelmsii and<br />Teucrium Stocksianum and screen them for possible analgesic activity<br />and to determine the safe dose range of total flavonoids for preclinical<br />studies in mice.<br /><strong>METHODS</strong>: Acute toxicity studies were performed in mice to determine<br />its safe dose range for total flavonoids of Achillea wilhelmsii and Teucrium<br />stocksianum. Writhing tests were performed in mice for possible<br />analgesic activity. Tail immersion method was used in rats for possible<br />analgesic activity.<br /><strong>RESULTS</strong>: Respective LD50 for crude flavonoids of Achillea wilhelmsii<br />(CFA) and crude flavonoids of Teucrium stocksianum (CFT) are<br />1312±12.76 mg/kg and 1248 ±29.6 mg/kg. In acetic acid induced<br />writhing, analgesic effect of CFA and CFT in dose 10 mg/kg is almost<br />comparable with standard acetylsalicylic acid (p≤0.05). Similarly, in tail<br />immersion method, reaction time was significantly prolonged in test<br />doses of 5 and 10 mg/kg (both for CFA and CFT) suggesting supraspinal<br />analgesic effect.<br /><strong>CONCLUSION</strong>: The results confirm that total crude flavonoids of both<br />the species, which are safe up to dose of 100 mg/kg, have analgesic<br />activity. It further warrants for isolation and purification of analgesic<br />flavonoids for new drug development.<br /><strong>KEY WORDS</strong>: Achillea (MeSH), Achillea wilhelmsii (Non-MeSH); Teucrium<br />stocksianum (Non-MeSH); flavonoids (MeSH), crude flavonoids<br />(Non-MeSH); Analgesics (MeSH).</p>}, number={1}, journal={KHYBER MEDICAL UNIVERSITY JOURNAL}, author={Ali, Niaz and Sultana, Ulfat and Ali Shah, Syed Wadood and Nabi, Muhammad and Ullah, Shakir and Shah, Ismail and Ahmad, Ghayour}, year={2016}, month={Apr.} }